10x tryple tm select enzyme for dissociation (Thermo Fisher)
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10x tryple tm select enzyme for dissociation
10x Tryple Tm Select Enzyme For Dissociation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple+tm+select+10x/PBS/pmc10405928-47-5-15
Average 99 stars, based on 1 article reviews
10x Tryple Tm Select Enzyme For Dissociation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple+tm+select+10x/PBS/pmc10405928-47-5-15
Average 99 stars, based on 1 article reviews
10x tryple tm select enzyme for dissociation - by Bioz Stars,
2026-10
99/100 stars
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Passaging:Article Title: Mechanistic Model of Signaling Dynamics Across an Epithelial Mesenchymal Transition Article Snippet: Cells were cultured at 37°C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2 . .. For cell passaging, cells were incubated with Incubation:Article Title: Mechanistic Model of Signaling Dynamics Across an Epithelial Mesenchymal Transition Article Snippet: Cells were cultured at 37°C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2 . .. For cell passaging, cells were incubated with Article Title: Mechanistic Model of Signaling Dynamics Across an Epithelial Mesenchymal Transition Article Snippet: .. For cell harvest, cells were washed twice with PBS and incubated with Article Title: Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51 Article Snippet: CRC-SCs were routinely maintained in Dulbecco’s Modified Eagle Medium (DMEM)/F12-based (#52100047, #21700018) culture medium specific for CSCs containing 4 mg/mL bovine serum albumin (BSA; #A1312, US Biological, Salem, MA), 1X Penicillin-Streptomycin-Amphotericin B (PSF; #17-745E, Lonza, Basel, Switzerland), 0.13% NaHCO3 (#25080094, Thermo Scientific), 6 mM Hepes (#15630056, Thermo Scientific), 2mM L-glutamine (#25030024, Thermo Scientific), 0.1 mg/mL apotransferrin (#T2252), 0.4 units heparin sodium salt (#H3393), 1.1% glucose (#G8769), 25 μg/mL insulin (#91077 C), 6.3 ng/mL progesterone (#P8783), 9.7 μg/mL putrescine dihydrochloride (#P5780), and 5.2 ng/mL sodium selenite (#S5261) and supplemented with 20 ng/mL human epidermal growth factor (EGF; #AF-100-15, PeproTech, London, UK), 10 ng/mL human basic fibroblast growth factor (bFGF; #AF-100-18B, PeproTech), and 10 mM nicotinamide (#N0636). .. All CRC-SCs including neo-resistant (neoR) CRC-SCs (whose generation is described in the next session) were passaged once/twice a week at dilution 1:2 by mechanical dissociation, through a micropipette, and/or enzymatic dissociation, by incubating them for less than 5 min at 37 °C with |